Publications

Immunology, Cancer & Glycomedicine

2025

Carneiro P, Vicente MM, Maria Isabel Leite, Santos M, Pinho SS, Fernandes Â. The role of N-glycans in regulatory T cells in autoimmunity. Autoimmunity Reviews 103791, ISSN 1568-9972 (2025) https://doi.org/10.1016/j.autrev.2025.103791

Pedro Almeida, Inês Alves, Ângela Fernandes, Cláudia Lima, Rui Freitas, Isaac Braga, Jorge Correia, Carmen Jerónimo, Salomé S. Pinho. Mannose glycans as key players in trained immunity: A novel anti-tumoral catalyst. Biochimica et Biophysica Acta 130779, ISSN 0304-4165 (2025) https://doi.org/10.1016/j.bbagen.2025.130779.

Rodrigues, CS., Gaifem, J., Pereira, MS, Alves, MF, Silva, M, Padrão, N, Cavadas, B, Moreira-Barbosa, C, Alves, I, Marcos-Pinto, R, Torres, J, Lavelle, A, Colombel, J-F, Sokol, H & Pinho, SS Alterations in mucosa branched N-glycans lead to dysbiosis and downregulation of ILC3: a key driver of intestinal inflammation. Gut Microbes 17, 2461210 (2025) https://doi.org/10.1080/19490976.2025.2461210

2024

Estevinho MM, Midya V, Cohen-Mekelburg S, Allin KH, Fumery M, Pinho SS, Colombel JF, Agrawal M. Emerging role of environmental pollutants in inflammatory bowel disease risk, outcomes and underlying mechanisms. Gut 2024. https://doi.org/10.1136/gutjnl-2024-332523

Almeida P, Fernandes Â, Alves I, Pinho SS. Glycans in Trained Immunity: Educators of innate immune memory in homeostasis and disease”. Carbohydr Res. 2024 544:109245 https://doi.org/10.1016/j.carres.2024.109245

Gaifem J*, Rodrigues CS*, Petralia F, Alves I, Leite-Gomes E, Cavadas B, Dias AM, Moreira-Barbosa C, Revés J, Laird RM, Novokmet M, Štambuk J, Habazin S, Turhan B, Gümüş ZH, Ungaro R, Torres J, Lauc G, Colombel JF, Porter CK, Pinho SS. A unique serum IgG glycosylation signature predicts development of Crohn’s disease and is associated with pathogenic antibodies to mannose glycan. Nat Immunol. 2024 25(9):1692-1703. https://doi.org/10.1038/s41590-024-01916-8

Lucy I. Crouch, Cláudia S. Rodrigues, Cassie R. Bakshani, Leticia Tavares-Gomes, Joana Gaifem, Salomé S. Pinho. The role of glycans in health and disease: Regulators of the interaction between gut microbiota and host immune system. Seminars in Immunology, Volume 73, 2024, 101891, ISSN 1044-5323 https://doi.org/10.1016/j.smim.2024.101891

A. S. Barros, S. Pinto, J. Viegas, C. Martins, H. Almeida, I. Alves, S. Pinho, R. Nunes, A. Harris, B. Sarmento. Orally Delivered Stimulus-Sensitive Nanomedicine to Harness Teduglutide Efficacy in Inflammatory Bowel Disease. Small 2024, 20, 2402502. https://doi.org/10.1002/smll.202402502

Henrique Fernandes-Mendes, Catarina M. Azevedo, Mónica Garrido, Carolina Lemos, Isabel Pedroto, Salomé S. Pinho, Ricardo Marcos-Pinto, Ângela Fernandes. Risk Factors in Serrated Pathway Lesions: N-Glycosylation Profile as a Potential Biomarker of Progression to Malignancy. GE Port J Gastroenterol 1 October 2024; 31 (5): 338–350 https://doi.org/10.1159/000535920

Gaifem J, Mendes-Frias A, Wolter M, Steimle A, Garzón MJ, Ubeda C, Nobre C, González A, Pinho SS, Cunha C, Carvalho A, Castro AG, Desai MS, Rodrigues F, Silvestre R. Akkermansia muciniphila and Parabacteroides distasonis synergistically protect from colitis by promoting ILC3 in the gut. mBio https://doi.org/10.1128/mbio.00078-24

2023

Vicente, M. M., Leite-Gomes, E., & Pinho, S. S. Glycome dynamics in T and B cell development: basic immunological mechanisms and clinical applications. Trends in immunology, 44(8), 585–597. https://doi.org/10.1016/j.it.2023.06.004

Pinho, S.S., Alves, I., Gaifem, J. et al. Immune regulatory networks coordinated by glycans and glycan-binding proteins in autoimmunity and infection. Cell Mol Immunol 20, 1101–1113 (2023). h ttps://doi.org/10.1038/s41423-023-01074-1

Campar, A., Alves, I., Santos-Pereira, B., Nogueira, R., Pinto, M. M., Vasconcelos, C., & Pinho, S. S. (2023). Muscle glycome in Idiopathic Inflammatory Myopathies: impact in IL-6 production and disease prognosis. iScience. https://www.sciencedirect.com/science/article/pii/S258900422301249X

Vicente, M. M., Alves, I., Fernandes, Â., Dias, A. M., Santos-Pereira, B., Pérez-Anton, E., … & Pinho, S. S. (2023). Mannosylated glycans impair normal T-cell development by reprogramming commitment and repertoire diversity. Cellular & Molecular Immunology, 1-14.https://doi.org/10.1038/s41423-023-01052-7

Alves, Inês; Santos-Pereira, Beatriz; de la Cruz, Noelia; Campar, Ana; Pinto, Vanda; Rodrigues, Pedro M.; Araújo, Marco; et al. “Host-derived mannose glycans trigger a pathogenic γd T cell/IL-17a axis in autoimmunity”. Science Translational Medicine 15 687 (2023): https://www.science.org/stoken/author-tokens/ST-1088/full

Ângela Fernandes; Catarina M. Azevedo; Mariana C. Silva; Guilherme Faria; Carolina S. Dantas; Manuel M. Vicente; Salomé S. Pinho. “Glycans as shapers of tumour microenvironment: A sweet driver of T-cell-mediated anti-tumour immune response”. Immunology (2023): https://doi.org/10.1111/imm.13494.

2022

Mortha, Arthur; Remark, Romain; Del Valle, Diane Marie; Chuang, Ling-Shiang; Chai, Zhi; Alves, Inês; Azevedo, Catarina; et al. “Neutralizing Anti-Granulocyte Macrophage-Colony Stimulating Factor Autoantibodies Recognize Post-Translational Glycosylations on Granulocyte Macrophage-Colony Stimulating Factor Years Before Diagnosis and Predict Complicated Crohn’s Disease”. Gastroenterology 163 3 (2022): 659-670. http://dx.doi.org/10.1053/j.gastro.2022.05.029.
10.1053/j.gastro.2022.05.029

Inês Alves*; Ângela Fernandes*; Beatriz Santos-Pereira; Catarina M. Azevedo; Salomé S. Pinho. “Glycans as a key factor in self and nonself discrimination: impact on the breach of immune tolerance”. FEBS Letters (2022): https://doi.org/10.1002/1873-3468.14347.

2021

Eduarda Leite-Gomes; Ana M Dias; Catarina M Azevedo; Beatriz Santos-Pereira; Mariana Magalhães; Mónica Garrido; Rita Amorim; et al. “Bringing to Light the Risk of Colorectal Cancer in Inflammatory Bowel Disease: Mucosal Glycosylation as a Key Player”. Inflammatory Bowel Diseases (2021): https://doi.org/10.1093/ibd/izab291.

Alves, Inês; Vicente, Manuel Machado; Gaifem, Joana; Fernandes, ngela; Dias, Ana Mendes; Rodrigues, Cláudia Sousa; Oliveira, José Carlos; et al. “SARS-CoV-2 Infection Drives a Glycan Switch of Peripheral T Cells at Diagnosis”. The Journal of Immunology 207 6 (2021): 1591-1598. http://dx.doi.org/10.4049/jimmunol.2100131

2020

Silva, Mariana C.; Fernandes, ngela; Oliveira, Maria; Resende, Carlos; Correia, Alexandra; de-Freitas-Junior, Julio C.; Lavelle, Aonghus; et al. “Glycans as Immune Checkpoints: Removal of Branched N-glycans Enhances Immune Recognition Preventing Cancer Progression”. Cancer Immunology Research 8 11 (2020): 1407-1425. http://dx.doi.org/10.1158/2326-6066.cir-20-0264.

Pereira, Márcia S.; Durães, Cecília; Catarino, Telmo A.; Costa, José L.; Cleynen, Isabelle; Novokmet, Mislav; Krištic, Jasminka; et al. “Genetic Variants of the MGAT5 Gene Are Functionally Implicated in the Modulation of T Cells Glycosylation and Plasma IgG Glycome Composition in Ulcerative Colitis”. Clinical and Translational Gastroenterology 11 4 (2020): e00166. http://dx.doi.org/10.14309/ctg.0000000000000166.

2019

Verhelst, Xavier; Dias, Ana M.; Colombel, Jean-Frederic; Vermeire, Severine; Van Vlierberghe, Hans; Callewaert, Nico; Pinho, Salomé S.. “protein glycosylation as a diagnostic and prognostic marker of chronic inflammatory gastrointestinal and liver diseases”. Gastroenterology (2019): http://dx.doi.org/10.1053/j.gastro.2019.08.060.

Pereira, M.S.; Maia, L.; Azevedo, L.F.; Campos, S.; Carvalho, S.; Dias, A.M.; Albergaria, A.; et al. “A [Glyco] biomarker that predicts failure to standard therapy in ulcerative colitis patients”. Journal of Crohn’s and Colitis 13 1 (2019): 39-49. http://www.scopus.com/inward/record.url?eid=2-s2.0-85057358772&partnerID=MN8TOARS http://www.scopus.com/inward/record.url?eid=2-s2.0-85057358772&partnerID=MN8TOARS.

2018

Rodrigues, J.G.; Balmaña, M.; Macedo, J.A.; Poças, J.; Fernandes, Â.; de-Freitas-Junior, J.C.M.; Pinho, S.S.; et al. “Glycosylation in cancer: Selected roles in tumour progression, immune modulation and metastasis”. Cellular Immunology (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85044285887&partnerID=MN8TOARS.

Dias, A.M.; Pereira, M.S.; Padrão, N.A.; Alves, I.; Marcos-Pinto, R.; Lago, P.; Pinho, S.S.. “Glycans as critical regulators of gut immunity in homeostasis and disease”. Cellular Immunology 333 (2018): 9-18. http://www.scopus.com/inward/record.url?eid=2-s2.0-85050201106&partnerID=MN8TOARS

Dias, A.M.; Correia, A.; Pereira, M.S.; Almeida, C.R.; Alves, I.; Pinto, V.; Catarino, T.A.; et al. “Metabolic control of T cell immune response through glycans in inflammatory bowel disease”. Proceedings of the National Academy of Sciences of the United States of America 115 20 (2018): E4651-E4660. http://www.scopus.com/inward/record.url?eid=2-s2.0-85046964805&partnerID=MN8TOARS

Klasic, M.; Markulin, D.; Vojta, A.; Samaržija, I.; Biruš, I.; Dobrinic, P.; Ventham, N.T.; et al. “Promoter methylation of the MGAT3 and BACH2 genes correlates with the composition of the immunoglobulin G glycome in inflammatory bowel disease”. Clinical Epigenetics 10 1 (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85048033567&partnerID=MN8TOARS

Pereira, M.S.; Alves, I.; Vicente, M.; Campar, A.; Silva, M.C.; Padrão, N.A.; Pinto, V.; et al. “Glycans as key checkpoints of T cell activity and function”. Frontiers in Immunology 9 NOV (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85057432575&partnerID=MN8TOARS

2017

Pinho, Salomé. “Glycans as Regulatory Elements of the Insulin/IGF System: Impact in Cancer Progression”. International Journal of Molecular Sciences (2017): http://www.mdpi.com/1422-0067/18/9/1921.

Pereira, C.C.; Durães, C.; Coelho, R.; Grácio, D.; Silva, M.; Peixoto, A.; Lago, P.; et al. “Association between polymorphisms in antioxidant genes and inflammatory bowel disease”. PLoS ONE 12 1 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85008394516 &partnerID=MN8TOAR

2016

Carvalho, S.; Catarino, T.A.; Dias, A.M.; Kato, M.; Almeida, A.; Hessling, B.; Figueiredo, J.; et al. “Preventing E-cadherin aberrant N-glycosylation at Asn-554 improves its critical function in gastric cancer”. Oncogene 35 13 (2016): 1619-1631. http://www.scopus.com/inward/record.url?eid=2-s2.0-84937605601&partnerID=MN8TOARS

Carvalho, S.; Reis, C.A.; Pinho, S.S.. “Cadherins Glycans in Cancer: Sweet Players in a Bitter Process”. Trends in Cancer 2 9 (2016): 519-531. http://www.scopus.com/inward/record.url?eid=2-s2.0-84989821862&partnerID=MN8TOARS

Carvalho, S.; Oliveira, T.; Bartels, M.F.; Miyoshi, E.; Pierce, M.; Taniguchi, N.; Carneiro, F.; et al. “O-mannosylation and N-glycosylation: Two coordinated mechanisms regulating the tumour suppressor functions of E-cadherin in cancer”. Oncotarget 7 40 (2016): 65231-65246. http://www.scopus.com/inward/record.url?eid=2-s2.0-84993660248&partnerID=MN8TOARS

2015

Pinho, S.S.; Reis, C.A.. “Glycosylation in cancer: Mechanisms and clinical implications”. Nature Reviews Cancer 15 9 (2015): 540-555. http://www.scopus.com/inward/record.url?eid=2-s2.0-84940449986&partnerID=MN8TOARS

2014

Dias, A.M.; Dourado, J.; Lago, P.; Cabral, J.; Marcos-Pinto, R.; Salgueiro, P.; Almeida, C.R.; et al. “Dysregulation of T cell receptor N-glycosylation: A molecular mechanism involved in ulcerative colitis”. Human Molecular Genetics 23 9 (2014): 2416-2427. http://www.scopus.com/inward/record.url?eid=2-s2.0-84897565140&partnerID=MN8TOARS

Bassagañas, S.; Carvalho, S.; Dias, A.M.; Pérez-Garay, M.; Ortiz, M.R.; Figueras, J.; Reis, C.A.; Pinho, S.S.; Peracaula, R.. “Pancreatic cancer cell glycosylation regulates cell adhesion and invasion through the modulation of a2ß1 integrin and E-cadherin function”. PLoS ONE 9 5 (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84902337828&partnerID=MN8TOARS.

Nunes, J.B.; Peixoto, J.; Soares, P.; Maximo, V.; Carvalho, S.; Pinho, S.S.; Vieira, A.F.; et al. “OXPHOS dysfunction regulates integrin-ß1 modifications and enhances cell motility and migration”. Human Molecular Genetics 24 7 (2014): 1977-1990. http://www.scopus.com/inward/record.url?eid=2-s2.0-84926488610&partnerID=MN8TOARS.

2013

De-Freitas-Junior, J.C.M.; Carvalho, S.; Dias, A.M.; Oliveira, P.; Cabral, J.; Seruca, R.; Oliveira, C.; et al. “Insulin/IGF-I signaling pathways enhances tumor cell invasion through bisecting GlcNAc N-glycans modulation. An interplay with E-cadherin”. PLoS ONE 8 11 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84896731020&partnerID=MN8TOARS

Pinho, S.S.; Figueiredo, J.; Cabral, J.; Carvalho, S.; Dourado, J.; Magalhães, A.; Gärtner, F.; et al. “E-cadherin and adherens-junctions stability in gastric carcinoma: Functional implications of glycosyltransferases involving N-glycan branching biosynthesis, N-acetylglucosaminyltransferases III and v”. Biochimica et Biophysica Acta – General Subjects 1830 3 (2013): 2690-2700. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873589547&partnerID=MN8TOARS

Pinho, S.S.; Carvalho, S.; Marcos-Pinto, R.; Magalhães, A.; Oliveira, C.; Gu, J.; Dinis-Ribeiro, M.; et al. “Gastric cancer: Adding glycosylation to the equation”. Trends in Molecular Medicine 19 11 (2013): 664-676. http://www.scopus.com/inward/record.url?eid=2-s2.0-84886951831&partnerID=MN8TOARS

2012

Paredes, J.; Figueiredo, J.; Albergaria, A.; Oliveira, P.; Carvalho, J.; Ribeiro, A.S.; Caldeira, J.; et al. “Epithelial E- and P-cadherins: Role and clinical significance in cancer”. Biochimica et Biophysica Acta – Reviews on Cancer 1826 2 (2012): 297-311. http://www.scopus.com/inward/record.url?eid=2-s2.0-84861800037&partnerID=MN8TOARS.

Pinho, S.S.; Oliveira, P.; Cabral, J.; Carvalho, S.; Huntsman, D.; Gärtner, F.; Seruca, R.; Reis, C.A.; Oliveira, C.. “Loss and recovery of Mgat3 and GnT-III mediated E-cadherin N-glycosylation is a mechanism involved in epithelial-Mesenchymal-Epithelial transitions”. PLoS ONE 7 3 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84858138360&partnerID=MN8TOARS

Pinho, S.S.; Carvalho, S.; Cabral, J.; Reis, C.A.; Gärtner, F.. “Canine tumors: A spontaneous animal model of human carcinogenesis”. Translational Research 159 3 (2012): 165-172. http://www.scopus.com/inward/record.url?eid=2-s2.0-84857276564&partnerID=MN8TOARS

2011

Klopfleisch, R.; von Euler, H.; Sarli, G.; Pinho, S.S.; Gärtner, F.; Gruber, A.D.. “Molecular carcinogenesis of canine mammary tumors: News from an old disease”. Veterinary Pathology 48 1 (2011): 98-116. http://www.scopus.com/inward/record.url?eid=2-s2.0-79951940506&partnerID=MN8TOARS

Pinho, S.S.; Seruca, R.; Gärtner, F.; Yamaguchi, Y.; Gu, J.; Taniguchi, N.; Reis, C.A.. “Modulation of E-cadherin function and dysfunction by N-glycosylation”. Cellular and Molecular Life Sciences 68 6 (2011): 1011-1020. http://www.scopus.com/inward/record.url?eid=2-s2.0-79953721653&partnerID=MN8TOARS

2009

Pinho, S.S.; Osório, H.; Nita-Lazar, M.; Gomes, J.; Lopes, C.; Gärtner, F.; Reis, C.A.. “Role of E-cadherin N-glycosylation profile in a mammary tumor model”. Biochemical and Biophysical Research Communications 379 4 (2009): 1091-1096. http://www.scopus.com/inward/record.url?eid=2-s2.0-58949097354&partnerID=MN8TOARS

Pinho, S.S.; Reis, C.A.; Paredes, J.; Magalhães, A.M.; Ferreira, A.C.; Figueiredo, J.; Xiaogang, W.; et al. “The role of N-acetylglucosaminyltransferase III and V in the post-transcriptional modifications of E-cadherin”. Human Molecular Genetics 18 14 (2009): 2599-2608. http://www.scopus.com/inward/record.url?eid=2-s2.0-67649859659&partnerID=MN8TOARS

Pinho, S.S.; Reis, C.A.; Gärtner, F.; Alpaugh, M.L.. “Molecular plasticity of E-cadherin and sialyl lewis X expression, in two comparative models of mammary tumorigenesis”. PLoS ONE 4 8 (2009): http://www.scopus.com/inward/record.url?eid=2-s2.0-68949159937&partnerID=MN8TOARS

de Oliveira, J.T.; Pinho, S.S.; de Matos, A.J.; Hespanhol, V.; Reis, C.A.; Gärtner, F.. “MUC1 expression in canine malignant mammary tumours and relationship to clinicopathological features”. Veterinary Journal 182 3 (2009): 491-493. http://www.scopus.com/inward/record.url?eid=2-s2.0-71849093863&partnerID=MN8TOARS

2007

Pinho, S.S.; Matos, A.J.F.; Lopes, C.; Marcos, N.T.; Carvalheira, J.; Reis, C.A.; Gärtner, F.. “Sialyl Lewis x expression in canine malignant mammary tumours: Correlation with clinicopathological features and E-Cadherin expression”. BMC Cancer 7 (2007): http://www.scopus.com/inward/record.url?eid=2-s2.0-34547799601&partnerID=MN8TOARS.